Tissue culture technicians use various techniques to asexually reproduce live tissue, under laboratory conditions, producing specimens that are genetically identical to, or different from, the parent tissue. The process of producing genetically identical specimens is commonly known as cloning. Tissue culture is used for a wide variety of commercially important agricultural and horticultural plants, in the propagation of rare and endangered plant species, and in the growth of tissues or cells used in health and medical research and treatments.
Duties & Tasks
Tissue culture technicians may perform the following tasks:clean, sterilise and maintain equipment and facilitiesset up and operate equipment including microscopes, environmental chambers (incubators) and centrifuges select live material with the desired characteristicsdissect and test tissues or cells for viruses and other diseasesprepare selected tissues or cells for reproductionprepare reproductive and growth chemical solutions (medias) suitable for the species to be clonedchemically treat selected tissues or cells to induce genetic mutationsplace tissues or cells in sterile chemical solutions or medias and place on shakers to initiate reproduction or growthplace reproduced plant material in sealed sterile containers containing a nutrient gel, then grow under controlled temperature and light conditions, repeating this process until the specimens are of sufficient sizegrow samples of cloned cells or organisms to maturity and monitor them for desirable or undesirable genetic mutations (genotypes) or characteristics (phenotypes)record results by using computers or other methods.
Tissue culture technicians mostly work in laboratories, with a small amount of field and shadehouse work.
- good eyesight (may be corrected) good hand-eye coordination able to concentrate on detail able to keep records accurately.